Vector-enabled metagenomic (VEM) surveys using whiteflies (Aleyrodidae) reveal novel begomovirus species in the New and Old Worlds

Simple item page
dc.contributor.authorRosario, K.
dc.contributor.authorSeah, Y.M.
dc.contributor.authorMarr, C.
dc.contributor.authorVarsani, A.
dc.contributor.authorKraberger, S.
dc.contributor.authorStainton, D.
dc.contributor.authorDuffy, S.
dc.contributor.authorBreitbart, M.
dc.contributor.authorMoriones, E.
dc.contributor.authorPolston, J.E.
dc.date.accessioned2016-06-21T02:38:48Z
dc.date.available2016-06-21T02:38:48Z
dc.date.issued2015en
dc.description.abstractWhitefly-transmitted viruses belonging to the genus Begomovirus (family Geminiviridae) represent a substantial threat to agricultural food production. The rapid evolutionary potential of these single-stranded DNA viruses combined with the polyphagous feeding behavior of their whitefly vector (Bemisia tabaci) can lead to the emergence of damaging viral strains. Therefore, it is crucial to characterize begomoviruses circulating in different regions and crops globally. This study utilized vector-enabled metagenomics (VEM) coupled with high-throughput sequencing to survey begomoviruses directly from whiteflies collected in various locations (California (USA), Guatemala, Israel, Puerto Rico, and Spain). Begomoviruses were detected in all locations, with the highest diversity identified in Guatemala where up to seven different species were identified in a single field. Both bipartite and monopartite viruses were detected, including seven new begomovirus species from Guatemala, Puerto Rico, and Spain. This begomovirus survey extends the known diversity of these highly damaging plant viruses. However, the new genomes described here and in the recent literature appear to reflect the outcome of interactions between closely-related species, often resulting from recombination, instead of unique, highly divergent species.en
dc.identifier.citationRosario, K., Seah, Y.M., Marr, C., Varsani, A., Kraberger, S., Stainton, D., Moriones, E. Polston, J.E., Duffy, S. and Breitbart, M. (2015) Vector-enabled metagenomic (VEM) surveys using whiteflies (Aleyrodidae) reveal novel begomovirus species in the New and Old Worlds. Viruses, 7, pp. 5553-5570.en
dc.identifier.doihttps://doi.org/10.3390/v7102895
dc.identifier.urihttp://hdl.handle.net/10092/12337
dc.language.isoen
dc.publisherUniversity of Canterbury. Biological Sciencesen
dc.publisherUniversity of Canterbury. Biomolecular Interaction Centreen
dc.rights.urihttps://hdl.handle.net/10092/17651
dc.subjectbegomovirusen
dc.subjectmetagenomicsen
dc.subjectwhiteflyen
dc.subjectvectoren
dc.subjectssDNAen
dc.subject.anzsrcField of Research::06 - Biological Sciencesen
dc.titleVector-enabled metagenomic (VEM) surveys using whiteflies (Aleyrodidae) reveal novel begomovirus species in the New and Old Worldsen
dc.typeJournal Article
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12657913_Rosario_2015.pdf
Size:
1.54 MB
Format:
Adobe Portable Document Format
Download
Collections
Science: Journal Articles