Novel Bi-Directional Dual-Flow-Rootchip to Study Effects of Osmotic Stress On Calcium Signalling in Arabidopsis Roots

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dc.contributor.authorAllan C
dc.contributor.authorTayagui A
dc.contributor.authorMeisrimler C-N
dc.contributor.authorNock, Volker
dc.date.accessioned2022-05-12T00:08:45Z
dc.date.available2022-05-12T00:08:45Z
dc.date.issued2022en
dc.date.updated2022-02-13T21:42:17Z
dc.description.abstractBeing able to detect and respond to abiotic and biotic stresses is fundamental for plant growth and survival. However, understanding of signal transduction within the root remains limited. To help shed light on these processes, we have developed a bidirectional-dual‐flow‐RootChip (bi-dfRC), which adds bidirectional stimulation to the existing asymmetric laminar flow root perfusion platforms. In this paper we show design, fabrication and characterisation of the bi-dfRC, as well as growth of wildtype and Ca2+ indicator (G-CaMP3) Arabidopsis thaliana plants on the platform. Applicability of the bi-dfRC is further demonstrated by probing the dynamic response of Arabidopsis roots to simulated drought stress effects via a fluorescent Ca2+ sensor in a variety of combinations and spatial orientations. The latter enables the tracking of growth, localisation, and quantity in response to bidirectional stimulation in real time at a cellular level.en
dc.identifier.citationAllan C, Tayagui A, Nock V, Meisrimler C-N (2022). Novel Bi-Directional Dual-Flow-Rootchip to Study Effects of Osmotic Stress On Calcium Signalling in Arabidopsis Roots. 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS). 09/01/2022-13/01/2022. 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS).en
dc.identifier.doihttp://doi.org/10.1109/mems51670.2022.9699700
dc.identifier.urihttps://hdl.handle.net/10092/103678
dc.language.isoenen
dc.publisherIEEEen
dc.rightsAll rights reserved unless otherwise stateden
dc.rights.urihttp://hdl.handle.net/10092/17651en
dc.subjectroot chipen
dc.subjectabiotic stressen
dc.subjectsalt stressen
dc.subjectsignal transductionen
dc.subjectcalcium signallingen
dc.subjectArabidopsisen
dc.subject.anzsrcFields of Research::31 - Biological sciences::3101 - Biochemistry and cell biology::310111 - Signal transductionen
dc.subject.anzsrcFields of Research::31 - Biological sciences::3108 - Plant biology::310803 - Plant cell and molecular biologyen
dc.titleNovel Bi-Directional Dual-Flow-Rootchip to Study Effects of Osmotic Stress On Calcium Signalling in Arabidopsis Rootsen
dc.typeConference Contributions - Publisheden
uc.collegeFaculty of Engineering
uc.departmentElectrical and Computer Engineering
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