Bacterial Contamination Assessment of Rural Ugandan Drinking Water Sources Using EC 3M TM petrifilms TM

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dc.contributor.authorPearson, A.L.
dc.contributor.authorLong, V.
dc.contributor.authorMeschke, J.S.
dc.contributor.authorRoberts, M.C.
dc.contributor.authorMayer, J.D.
dc.date.accessioned2010-05-27T22:16:36Z
dc.date.available2010-05-27T22:16:36Z
dc.date.issued2007en
dc.description.abstractThis study examined bacterial contamination levels from 47drinking water sources(92%were surface sources)from two rural Ugandan villages using EC 3M TM petrifilmsTM incubated for 24 h at 37ÂșC. E. coli counts in 1ml water samples ranged from 0-120 cfu/ml. Confirmation of presumptive E. coli was performed by transferring colonies onto Blood agar followed by characterization by subculture on selective media and standard biochemical analysis. Selected isolates were verified by sequencing of their 16S rRNA genes. A total of 184 colonies were isolated from petrifilms representing 70%of the water sources and 92% of the isolates were identified to genusl evel (n=169). Only 24%of presumptive E. coli, isolates were confirmed as E. coli (n=40). Additionally 61%of isolates were found to be lactose negative and were confirmed as Salmonella, Yersinia and Proteus (n=103). Purified bacterial isolates from the Ugandan water samples and laboratory strains were replated on new petrifilms at various dilutions to determine the potential for misclassification of colony color or gas production. Upon inoculation of new petrifilms, misclassification of coliforms was observed. While the level of bacterial contamination in the rural Ugandan drinking water sources is not unexpected, isolation of lactose negative bacteria from presumptive E. coli colonies and misclassification of coliforms by the petrifilms was unexpected.en
dc.identifier.citationPearson, A.L., Long, V., Meschke, J.S., Roberts, M.C., Mayer, J.D. (2007) Bacterial Contamination Assessment of Rural Ugandan Drinking Water Sources Using EC 3M TM petrifilms TM. Toronto, Canada: 107th American Society for Microbiology Conference, 21-25 May 2007.en
dc.identifier.urihttp://hdl.handle.net/10092/3904
dc.language.isoen
dc.publisherUniversity of Canterbury. Geographyen
dc.rights.urihttps://hdl.handle.net/10092/17651en
dc.subject.marsdenFields of Research::320000 Medical and Health Sciences::320400 Medical Microbiology::320403 Medical infection agents (incl. prions)en
dc.titleBacterial Contamination Assessment of Rural Ugandan Drinking Water Sources Using EC 3M TM petrifilms TMen
dc.typeConference Contributions - Other
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