Environmental Enrichment Elicits a Transient Rise of Bioactive C-Type Natriuretic Peptide in Young but Not Aged Rats

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dc.contributor.authorRapley SA
dc.contributor.authorPrickett TCR
dc.contributor.authorEspiner EA
dc.contributor.authorDalrymple-Alford, John
dc.date.accessioned2021-07-04T23:43:16Z
dc.date.available2021-07-04T23:43:16Z
dc.date.issued2018en
dc.date.updated2021-06-29T03:28:11Z
dc.description.abstractBeneficial molecular and neuroplastic changes have been demonstrated in response to environmental enrichment (EE) in laboratory animals across the lifespan. Here, we investigated whether these effects extend to C-type Natriuretic Peptide (CNP), a widely expressed neuropeptide with putative involvement in neuroprotection, neuroplasticity, anxiety, and learning and memory. We determined the CNP response in 36 young (8-9 months) and 36 aged (22-23 months) male PVGc hooded rats that were rehoused with new cage mates in either standard laboratory cages or EE for periods of 14 or 28 days. Tissues were rapidly excised from four brain regions associated with memory formation (dorsal hippocampus, retrosplenial cortex, medial prefrontal cortex, and mammillary bodies) plus the occipital cortex and hypothalamus, and immediately frozen. Radioimmunoassay was used to measure bioactive CNP and the amino-terminal fragment of proCNP, NTproCNP. Because CNP but not NTproCNP is rapidly degraded at source, NTproCNP reflects CNP production whereas the ratio NTproCNP:CNP is a biomarker of CNP’s local degradation rate. EE increased CNP at 14 days in all brain regions in young, but not old rats; this effect in young rats was lost at 28 days in all regions of interest. NTproCNP:CNP ratio, but not NTproCNP, was reduced in all regions by EE at 14 days in young rats, but not in old rats, which suggests a period of reduced degradation or receptor mediated clearance, rather than increased production of CNP in these young EE rats. Aged rats tended to show reduced NTproCNP:CNP ratios but this did not occur in dorsal hippocampus or mammillary bodies. This is the first study demonstrating modulation of CNP protein concentrations, and the effect of age, in response to environmental stimulation. Furthermore, it is the first to show that changes in degradation rate in vivo may be an important component in determining CNP bioactivity in neural tissues.en
dc.identifier.citationRapley SA, Prickett TCR, Dalrymple-Alford JC, Espiner EA (2018). Environmental Enrichment Elicits a Transient Rise of Bioactive C-Type Natriuretic Peptide in Young but Not Aged Rats. Frontiers in Behavioral Neuroscience. 12:142.en
dc.identifier.doihttp://doi.org/10.3389/fnbeh.2018.00142
dc.identifier.issn1662-5153
dc.identifier.urihttps://hdl.handle.net/10092/102164
dc.languageeng
dc.language.isoen
dc.publisherFrontiers Media SAen
dc.rightsCopyright © 2018 Rapley, Prickett, Dalrymple-Alford and Espiner. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://hdl.handle.net/10092/17651en
dc.subjectC-type natriuretic peptideen
dc.subjectenvironmental enrichmenten
dc.subjectagingen
dc.subjectmedial prefrontal cortexen
dc.subjecthippocampusen
dc.subjectretrosplenial cortexen
dc.subject.anzsrc1109 Neurosciencesen
dc.subject.anzsrc1701 Psychologyen
dc.subject.anzsrc1702 Cognitive Sciencesen
dc.subject.anzsrcFields of Research::31 - Biological sciences::3109 - Zoology::310906 - Animal neurobiologyen
dc.subject.anzsrcFields of Research::52 - Psychology::5202 - Biological psychology::520203 - Cognitive neuroscienceen
dc.subject.anzsrcFields of Research::52 - Psychology::5204 - Cognitive and computational psychology::520403 - Learning, motivation and emotionen
dc.titleEnvironmental Enrichment Elicits a Transient Rise of Bioactive C-Type Natriuretic Peptide in Young but Not Aged Ratsen
dc.typeJournal Articleen
uc.collegeFaculty of Science
uc.departmentSchool of Psychology, Speech and Hearing
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