Herbicide ingredients change Salmonella enterica sv. Typhimurium and Escherichia coli antibiotic responses

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dc.contributor.authorKurenbach B
dc.contributor.authorGibson PS
dc.contributor.authorHill AM
dc.contributor.authorBitzer AS
dc.contributor.authorSilby MW
dc.contributor.authorGodsoe W
dc.contributor.authorHeinemann, J
dc.date.accessioned2018-12-03T23:38:32Z
dc.date.available2018-12-03T23:38:32Z
dc.date.issued2017en
dc.date.updated2018-10-31T19:34:53Z
dc.description.abstract© 2017 The Authors. Herbicides are frequently released into both rural and urban environments. Commercial herbicide formulations induce adaptive changes in the way bacteria respond to antibiotics. Salmonella enterica sv. Typhimurium and Escherichia coli were exposed to common co-formulants of formulations, and S. enterica sv. Typhimurium was exposed to active ingredients dicamba, 2,4-D and glyphosate to determine what ingredients of the commercial formulations caused this effect. Coformulants Tween80 and carboxymethyl cellulose induced changes in response, but the pattern of the responses differed from the active ingredients, and effect sizes were smaller. A commercial wetting agent did not affect antibiotic responses. Active ingredients induced changes in antibiotic responses similar to those caused by complete formulations. This occurred at or below recommended application concentrations. Targeted deletion of efflux pump genes largely neutralized the adaptive response in the cases of increased survival in antibiotics, indicating that the biochemistry of induced resistance was the same for formulations and specific ingredients. We found that glyphosate, dicamba, and 2,4-D, as well as co-formulants in commercial herbicides, induced a change in susceptibility of the potentially pathogenic bacteria E. coli and S. enterica to multiple antibiotics. This was measured using the efficiency of plating (EOP), the relative survival of the bacteria when exposed to herbicide and antibiotic, or just antibiotic, compared to survival on permissive media. This work will help to inform the use of non-medicinal chemical agents that induce changes in antibiotic responses.en
dc.identifier.doihttps://doi.org/10.1099/mic.0.000573
dc.identifier.issn1350-0872
dc.identifier.issn1465-2080
dc.identifier.urihttp://hdl.handle.net/10092/16268
dc.languageEnglish
dc.language.isoen
dc.rightsThis is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.en
dc.subjectpesticidesen
dc.subjectantibioticsen
dc.subjectantibiotic resistant bacteriaen
dc.subject.anzsrcFields of Research::31 - Biological sciences::3107 - Microbiology::310701 - Bacteriologyen
dc.subject.anzsrcField of Research::11 - Medical and Health Sciences::1108 - Medical Microbiology::110801 - Medical Bacteriologyen
dc.subject.anzsrcFields of Research::31 - Biological sciences::3107 - Microbiology::310703 - Microbial ecologyen
dc.subject.anzsrcField of Research::07 - Agricultural and Veterinary Sciences::0799 - Other Agricultural and Veterinary Sciences::079902 - Fertilisers and Agrochemicals (incl. Application)en
dc.titleHerbicide ingredients change Salmonella enterica sv. Typhimurium and Escherichia coli antibiotic responsesen
dc.typeJournal Articleen
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