Suspended Cell Patterning for Automatic Microrobotic Cell Injection

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dc.contributor.authorWang, W.H.
dc.contributor.authorAlkaisi, M.M.
dc.contributor.authorLiu, X.Y.
dc.contributor.authorSun, Y.
dc.contributor.authorChase, Geoff
dc.contributor.authorChen, X.Q.
dc.contributor.authorHann, C.E.
dc.date.accessioned2009-04-23T00:44:14Z
dc.date.available2009-04-23T00:44:14Z
dc.date.issued2008en
dc.description.abstractMicroinjection of DNA/mRNA/morpholinos is a critical technology for molecular biology and drug discovery. When dealing with suspended cells, state-of-the-art manual injection involves a time-consuming and tedious sample preparation procedure, to accurately align cells. To enable automatic microrobotic cell injection, this paper reports on two inexpensive, reusable, biocompatible, and easy-to-make devices that are capable of patterning a large number of cells in 10-30 seconds. One device is based on negative air pressure and made of polycarbonate using a conventional micro-machining process. It is particularly suitable for cells larger than 100¿m, such as the zebrafish embryo patterning and successful gene 'knock-down' products of the morpholino-injected embryos. The other device is based on dielectrophoresis and suitable for cells smaller than 100¿m, demonstrated by successful trapping of pituitary cells. These devices offer a complete solution for suspended cells in all size spectrums to be prepared up to 10 times faster than manual human preparation. Furthermore, this approach can facilitate high-throughput automatic microrobotic cell injection, for injection applications such as the injection of zebrafish embryos, mouse oocytes/embryos, Drosophila embryos, and other types of suspended cells.en
dc.identifier.citationWang, W.H., Alkaisi, M.M., Liu, X.Y., Sun, Y., Chase, J.G., Chen, X.Q., Hann, C.E. (2008) Suspended Cell Patterning for Automatic Microrobotic Cell Injection. Beijing, China: IEEE/ASME International Conference on Mechatronic and Embedded Systems and Applications 2008 (MESA 2008), 12-15 Oct 2008. 100-105.en
dc.identifier.doihttps://doi.org/10.1109/MESA.2008.4735648
dc.identifier.urihttp://hdl.handle.net/10092/2347
dc.language.isoen
dc.publisherUniversity of Canterbury. Mechanical Engineering.en
dc.rights.urihttps://hdl.handle.net/10092/17651en
dc.subjectcell injectionen
dc.subjectcell patterningen
dc.subjectfluidic deviceen
dc.subjectdielectrophoresisen
dc.subjectmicroroboticsen
dc.subject.marsdenFields of Research::270000 Biological Sciences::270100 Biochemistry and Cell Biologyen
dc.subject.marsdenFields of Research::290000 Engineering and Technology::290500 Mechanical and Industrial Engineering::290501 Mechanical engineeringen
dc.titleSuspended Cell Patterning for Automatic Microrobotic Cell Injectionen
dc.typeConference Contributions - Published
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