A micro-device integrating vertical and bottom electrodes for 3D cell rotation

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dc.contributor.authorBenhal, P.
dc.contributor.authorChase, Geoff
dc.contributor.authorGaynor, P.
dc.contributor.authorOback, B.
dc.contributor.authorWang, W.
dc.date.accessioned2014-11-18T01:27:36Z
dc.date.available2014-11-18T01:27:36Z
dc.date.issued2013en
dc.description.abstractWe report a Lab-on-chip micro device for rotating a spherical object such as bovine cell in three dimensional (3D) spaces. Micro device consists of two pairs of orthogonal vertical wall electrode along with bottom transparent indium tin oxide (ITO) electrodes. Photolithography technique is employed to fabricate bottom part of the micro device. On the other hand micro milling is applied to fabricate the top electrodes of 500 μm height. The entire device is only 75X25 mm in length and width upon which particle rotation is achieved. Micro device works entirely on this dielectrophoresis (DEP) phenomenon to rotate the cell in 3D spaces. Rotating fields are optimized through simulation and results are presented along with analysis. The experiments are carried out to determine the cell rotation rate. Rotation of cell in 3D spaces has its unique advantages in automation of high throughput mammalian cloning procedures. Cloning procedures such as enucleation requires a cell to be rotated in 3D spaces for genetic material extraction. Enucleation also requires the cell to be precisely rotated and controlled. This precise rotation control is achieved by electrorotation through DEP.en
dc.identifier.citationBenhal, P., Chase, J.G., Gaynor, P., Oback, B., Wang, W. (2013) A micro-device integrating vertical and bottom electrodes for 3D cell rotation. Auckland, New Zealand: 6th International Conference on Advanced Materials and Technology (AMN6), 11-15 Feb 2013. 1pp.en
dc.identifier.urihttp://hdl.handle.net/10092/9908
dc.language.isoen
dc.publisherUniversity of Canterbury. Electrical and Computer Engineeringen
dc.publisherUniversity of Canterbury. Mechanical Engineeringen
dc.rights.urihttps://hdl.handle.net/10092/17651en
dc.subjectDEPen
dc.subjectelectrorotationen
dc.subjectmicro-deviceen
dc.subjectmicro-millingen
dc.subject.anzsrcFields of Research::40 - Engineering::4003 - Biomedical engineering::400305 - Biomedical instrumentationen
dc.subject.anzsrcFields of Research::40 - Engineering::4014 - Manufacturing engineering::401410 - Microtechnologyen
dc.subject.anzsrcFields of Research::40 - Engineering::4017 - Mechanical engineering::401705 - Microelectromechanical systems (MEMS)en
dc.subject.anzsrcFields of Research::32 - Biomedical and clinical sciences::3208 - Medical physiology::320801 - Cell physiologyen
dc.titleA micro-device integrating vertical and bottom electrodes for 3D cell rotationen
dc.typeConference Contributions - Other
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