The Avon Stream arises from one helocrene and four rheocrene Springs. These have a constant flow into a small moderately fast stream with a bed of clay, mud and patches of weed. Oxygen, temperature, pH, cross sectional area and flow were measured in conjunction with biological sampling. Oxygen Saturation of the spring water was constant at 51% while downstream it showed a diel fluctuation. Animal density ranged from 100-500/0.1 m^2. Oligochaeta, Amphipoda and Mollusca were the most abundant faunal groups while Platyhelminthes were noticeably less common. A dilution technique for increasing counting accuracy is described. A biotope for the spring stream is proposed.