Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background grapheme

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dc.contributor.authorSeuring C
dc.contributor.authorAyyer K
dc.contributor.authorFilippaki E
dc.contributor.authorBarthelmess M
dc.contributor.authorLongchamp JN
dc.contributor.authorRingler P
dc.contributor.authorPardini T
dc.contributor.authorWojtas DH
dc.contributor.authorColeman MA
dc.contributor.authorDörner K
dc.contributor.authorFuglerud S
dc.contributor.authorHammarin G
dc.contributor.authorHabenstein B
dc.contributor.authorLangkilde AE
dc.contributor.authorLoquet A
dc.contributor.authorMeents A
dc.contributor.authorRiek R
dc.contributor.authorStahlberg H
dc.contributor.authorBoutet S
dc.contributor.authorHunter MS
dc.contributor.authorKoglin J
dc.contributor.authorLiang M
dc.contributor.authorGinn HM
dc.contributor.authorMillane RP
dc.contributor.authorFrank M
dc.contributor.authorBarty A
dc.contributor.authorChapman HN
dc.date.accessioned2018-11-18T23:06:05Z
dc.date.available2018-11-18T23:06:05Z
dc.date.issued2018en
dc.date.updated2018-10-31T19:34:24Z
dc.description.abstract© 2018 The Author(s). Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.en
dc.identifier.doihttps://doi.org/10.1038/s41467-018-04116-9
dc.identifier.issn2041-1723
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/10092/16228
dc.languageEnglish
dc.language.isoen
dc.rightsOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.en
dc.subject.anzsrcFields of Research::31 - Biological sciences::3101 - Biochemistry and cell biology::310109 - Proteomics and intermolecular interactions (excl. medical proteomics)en
dc.subject.anzsrcField of Research::06 - Biological Sciences::0601 - Biochemistry and Cell Biology::060102 - Bioinformaticsen
dc.subject.anzsrcFields of Research::31 - Biological sciences::3101 - Biochemistry and cell biology::310112 - Structural biology (incl. macromolecular modelling)en
dc.titleFemtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphemeen
dc.typeJournal Articleen
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