Generation of c-MycER<sup>TAM</sup>-transduced human late-adherent olfactory mucosa cells for potential regenerative applications

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dc.contributor.authorSantiago-Toledo , Gerardo
dc.contributor.authorGeorgiou , Melanie
dc.contributor.authordos Reis , Joana
dc.contributor.authorRoberton , Victoria H.
dc.contributor.authorValinhas , Ana
dc.contributor.authorWood, Rachael
dc.contributor.authorPhillips , James B.
dc.contributor.authorMason , Chris
dc.contributor.authorLi , Daqing
dc.contributor.authorLi , Ying
dc.contributor.authorSinden , John D.
dc.contributor.authorChoi , David
dc.contributor.authorJat , Parmjit S.
dc.contributor.authorWall , Ivan B.
dc.date.accessioned2025-02-09T20:53:32Z
dc.date.available2025-02-09T20:53:32Z
dc.date.issued2019
dc.description.abstractHuman olfactory mucosa cells (hOMCs) have been transplanted to the damaged spinal cord both pre-clinically and clinically. To date mainly autologous cells have been tested. However, inter-patient variability in cell recovery and quality, and the fact that the neuroprotective olfactory ensheathing cell (OEC) subset is difficult to isolate, means an allogeneic hOMC therapy would be an attractive “off-the-shelf” alternative. The aim of this study was to generate a candidate cell line from late-adherent hOMCs, thought to contain the OEC subset. Primary late-adherent hOMCs were transduced with a c-MycERTAM gene that enables cell proliferation in the presence of 4-hydroxytamoxifen (4-OHT). Two c-MycERTAM-derived polyclonal populations, PA5 and PA7, were generated and expanded. PA5 cells had a normal human karyotype (46, XY) and exhibited faster growth kinetics than PA7, and were therefore selected for further characterisation. PA5 hOMCs express glial markers (p75NTR, S100ß, GFAP and oligodendrocyte marker O4), neuronal markers (nestin and ß-III-tubulin) and fibroblast-associated markers (CD90/Thy1 and fibronectin). Co-culture of PA5 cells with a neuronal cell line (NG108-15) and with primary dorsal root ganglion (DRG) neurons resulted in significant neurite outgrowth after 5 days. Therefore, c-MycERTAM-derived PA5 hOMCs have potential as a regenerative therapy for neural cells.
dc.identifier.citationSantiago-Toledo G, Georgiou M, dos Reis J, Roberton VH, Valinhas A, Wood RC, Phillips JB, Mason C, Li D, Li Y, Sinden JD, Choi D, Jat PS, Wall IB (2019). Generation of c-MycER<sup>TAM</sup>-transduced human late-adherent olfactory mucosa cells for potential regenerative applications. Scientific Reports. 9(1). 13190-.
dc.identifier.doihttp://doi.org/10.1038/s41598-019-49315-6
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10092/107423
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAll rights reserved unless otherwise stated
dc.rights.urihttp://hdl.handle.net/10092/17651
dc.subjectOlfactory Mucosa
dc.subjectGanglia, Spinal
dc.subjectCell Line
dc.subjectRats, Sprague-Dawley
dc.subjectNeuroblastoma
dc.subjectTamoxifen
dc.subjectGentamicins
dc.subjectReceptors, Estrogen
dc.subjectRecombinant Proteins
dc.subjectCoculture Techniques
dc.subjectKaryotyping
dc.subjectTransduction, Genetic
dc.subjectGenes, myc
dc.subjectTransgenes
dc.subjectSensory Receptor Cells
dc.subjectBiomarkers
dc.subject.anzsrc32 - Biomedical and clinical sciences::3209 - Neurosciences::320902 - Cellular nervous system
dc.subject.anzsrc32 - Biomedical and clinical sciences::3209 - Neurosciences::320905 - Neurology and neuromuscular diseases
dc.titleGeneration of c-MycER<sup>TAM</sup>-transduced human late-adherent olfactory mucosa cells for potential regenerative applications
dc.typeJournal Article
uc.collegeFaculty of Engineering
uc.departmentChemical and Process Engineering
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