Enzyme Kinetics Analysis (EKA): an online tool for analysing enzyme initial rate data and teaching enzyme kinetics

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dc.contributor.authorMak D
dc.contributor.authorDunn S
dc.contributor.authorCoombes D
dc.contributor.authorCarere C
dc.contributor.authorAllison JR
dc.contributor.authorNock, Volker
dc.contributor.authorHudson A
dc.contributor.authorDobson R
dc.date.accessioned2024-07-04T01:03:10Z
dc.date.available2024-07-04T01:03:10Z
dc.date.issued2024
dc.description.abstractEnzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and cellular biology to pharmacology. However, because enzyme kinetics involves concepts rarely employed in other areas of biology, it can be challenging for students and researchers. Traditional graphical analysis was replaced by computational analysis, requiring another skill not core to many life sciences curricula. Computational analysis can be time‐consuming and difficult in free software (e.g., R) or require costly software (e.g., GraphPad Prism). We present Enzyme Kinetics Analysis (EKA), a web‐tool to augment teaching and learning and streamline EKA. EKA is an interactive and free tool for analyzing enzyme kinetic data and improving student learning through simulation, built using R and RStudio's ShinyApps. EKA provides kinetic models (Michaelis–Menten, Hill, simple reversible inhibition models, ternary‐complex, and ping‐pong) for users to fit experimental data, providing graphical results and statistics. Additionally, EKA enables users to input parameters and create data and graphs, to visualize changes to parameters (e.g., or number of measurements). This function is designed for students learning kinetics but also for researchers to design experiments. EKA provides a simple, interactive interface for teachers, students, and researchers to explore enzyme kinetics. It gives researchers the ability to design experiments and analyze data without specific software requirements.
dc.identifier.citationMak D, Dunn S, Coombes D, Carere C, Allison JR, Nock V, Hudson A, Dobson R (2024). Enzyme Kinetics Analysis (EKA): an online tool for analysing enzyme initial rate data and teaching enzyme kinetics. Biochemistry and Molecular Biology Education. 1-11.
dc.identifier.doihttp://doi.org/10.1002/bmb.21823
dc.identifier.issn1470-8175
dc.identifier.issn1539-3429
dc.identifier.urihttps://hdl.handle.net/10092/106750
dc.languageen
dc.publisherWiley
dc.rightsAll rights reserved unless otherwise stated
dc.rights.urihttp://hdl.handle.net/10092/17651
dc.subjectcomputational modeling
dc.subjectenzyme kinetics
dc.subjectonline analysis
dc.subjectR
dc.subjectteaching
dc.subject.anzsrc31 - Biological sciences::3101 - Biochemistry and cell biology::310106 - Enzymes
dc.subject.anzsrc31 - Biological sciences::3101 - Biochemistry and cell biology::310199 - Biochemistry and cell biology not elsewhere classified
dc.subject.anzsrc39 - Education::3901 - Curriculum and pedagogy::390113 - Science, technology and engineering curriculum and pedagogy
dc.subject.anzsrc39 - Education::3904 - Specialist studies in education::390405 - Educational technology and computing
dc.subject.anzsrc31 - Biological sciences::3102 - Bioinformatics and computational biology::310299 - Bioinformatics and computational biology not elsewhere classified
dc.titleEnzyme Kinetics Analysis (EKA): an online tool for analysing enzyme initial rate data and teaching enzyme kinetics
dc.typeJournal Article
uc.collegeFaculty of Engineering
uc.departmentElectrical and Computer Engineering
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