Construction with composite materials has become increasingly popular in contemporary structural design for multi-storey residential, commercial, and industrial buildings. As a composite structure, pre-stressed heavy timber buildings offer sustainable, environmentally-friendly advantages over competing construction technologies utilising structural steel and concrete components. Research at the University of Canterbury is continually investigating the performance and behaviour of this composite heavy timber construction assembly. The following research report provides a fire resistance analysis for pre-stressed heavy timber structures that includes: • A comprehensive literature review detailing the fire resistance for pre-stressed heavy timber structural components and typical connections; and • A four-phase series of experiments with epoxy grouted steel threaded rods and proprietary mechanical fasteners to determine the fire resistance properties of steel to wood connections. Laboratory experimentation includes cold testing to determine connection performance at ambient temperature, oven testing to evaluate heating effects on steel to wood connections, cooled testing to determine the residual strength of connections in minor fires and, finally, furnace testing to generate fire resistance design and analysis equations to be utilised for steel to wood connections. Recommendations for the fire performance of connections in pre-stressed heavy timber structures are included in the report.