Analysis of XFEL serial diffraction data from individual crystalline fibrils

Type of content
Journal Article
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Volume Title
Language
English
Date
2017
Authors
Wojtas DH
Ayyer K
Liang M
Mossou E
Romoli F
Seuring C
Beyerlein KR
Bean RJ
Morgan AJ
Oberthuer D
Abstract

Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.

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Keywords
serial crystallography, coherent X-ray diffractive imaging (CXDI), single particles, molecular orientation determination, crystalline fibrils, amyloid
Ngā upoko tukutuku/Māori subject headings
ANZSRC fields of research
Fields of Research::51 - Physical sciences::5102 - Atomic, molecular and optical physics::510202 - Lasers and quantum electronics
Field of Research::04 - Earth Sciences::0403 - Geology::040306 - Mineralogy and Crystallography
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