SDT: A Virus Classification Tool Based on Pairwise Sequence Alignment and Identity Calculation

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dc.contributor.authorMuhire, B.M.
dc.contributor.authorVarsani, A.
dc.contributor.authorMartin, D.P.
dc.date.accessioned2014-10-28T04:00:54Z
dc.date.available2014-10-28T04:00:54Z
dc.date.issued2014en
dc.description.abstractThe perpetually increasing rate at which viral full-genome sequences are being determined is creating a pressing demand for computational tools that will aid the objective classification of these genome sequences. Taxonomic classification approaches that are based on pairwise genetic identity measures are potentially highly automatable and are progressively gaining favour with the International Committee on Taxonomy of Viruses (ICTV). There are, however, various issues with the calculation of such measures that could potentially undermine the accuracy and consistency with which they can be applied to virus classification. Firstly, pairwise sequence identities computed based on multiple sequence alignments rather than on multiple independent pairwise alignments can lead to the deflation of identity scores with increasing dataset sizes. Also, when gap-characters need to be introduced during sequence alignments to account for insertions and deletions, methodological variations in the way that these characters are introduced and handled during pairwise genetic identity calculations can cause high degrees of inconsistency in the way that different methods classify the same sets of sequences. Here we present Sequence Demarcation Tool (SDT), a free user-friendly computer program that aims to provide a robust and highly reproducible means of objectively using pairwise genetic identity calculations to classify any set of nucleotide or amino acid sequences. SDT can produce publication quality pairwise identity plots and colour-coded distance matrices to further aid the classification of sequences according to ICTV approved taxonomic demarcation criteria. Besides a graphical interface version of the program for Windows computers, command-line versions of the program are available for a variety of different operating systems (including a parallel version for cluster computing platforms).en
dc.identifier.citationMuhire, B.M., Varsani, A., Martin, D.P. (2014) SDT: A Virus Classification Tool Based on Pairwise Sequence Alignment and Identity Calculation. PLoS One, 9(9), pp. e108277.en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0108277
dc.identifier.urihttp://hdl.handle.net/10092/9750
dc.language.isoen
dc.publisherUniversity of Canterbury. Biological Sciencesen
dc.publisherUniversity of Canterbury. Biomolecular Interaction Centreen
dc.rights.urihttps://hdl.handle.net/10092/17651en
dc.subject.anzsrcFields of Research::31 - Biological sciences::3107 - Microbiology::310706 - Virologyen
dc.subject.anzsrcFields of Research::31 - Biological sciences::3105 - Genetics::310508 - Genome structure and regulationen
dc.titleSDT: A Virus Classification Tool Based on Pairwise Sequence Alignment and Identity Calculationen
dc.typeJournal Article
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