Yoon, C.H.Bones, P.J.Millane, R.P.2010-01-122010-01-122005Yoon, C.H., Bones, P.J. and Millane, R.P. (2005) Image analysis for electron microscopy of muscle fibres. Cairns, Australia: Digital Image Computing: Techniques and Applications, 6-8 Dec 2005. Proceedings, CD-Rom, 79/1-7.http://hdl.handle.net/10092/3351An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined by filtering with a point spread function that incorporates the local symmetry in an image. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and the orientations classified using a Gaussian mixture model. Application to micrographs and comparison with manual classification of orientations shows that the system is effective.en©2005 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.Conference Contributions - PublishedFields of Research::290000 Engineering and Technology::290900 Electrical and Electronic Engineering::290903 Other electronic engineeringFields of Research::320000 Medical and Health Sciences::321000 Clinical Sciences::321022 Radiology and organ imagingFields of Research::320000 Medical and Health Sciences::329900 Other Medical and Health Sciences::329902 Medical biotechnology