Van Doorslaer KKraberger SAustin CFarkas KBergeman MPaunil EDavison WVarsani A2018-11-182018-11-1820180022-13171465-2099http://hdl.handle.net/10092/16229The Polyomaviridae is a diverse family of circular double-stranded DNA viruses. Polyomaviruses have been isolated from a wide array of animal hosts. An understanding of the evolutionary and ecological dynamics of these viruses is essential to understanding the pathogenicity of polyomaviruses. Using a high throughput sequencing approach, we identified a novel polyomavirus in an emerald notothen (Trematomus bernacchii) sampled in the Ross sea (Antarctica), expanding the known number of fish-associated polyomaviruses. Our analysis suggests that polyomaviruses belong to three main evolutionary clades; the first clade is made up of all recognized terrestrial polyomaviruses. The fish-associated polyomaviruses are not monophyletic, and belong to two divergent evolutionary lineages. The fish viruses provide evidence that the evolution of the key viral large T protein involves gain and loss of distinct domains.enCopyright 2018 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.PolyomaviridaeAntarcticaemerald notothenTrematomus bernacchiiJournal Article2018-10-31Fields of Research::31 - Biological sciences::3107 - Microbiology::310706 - VirologyFields of Research::31 - Biological sciences::3104 - Evolutionary biology::310410 - Phylogeny and comparative analysishttps://doi.org/10.1099/jgv.0.001041