A micro-device integrating vertical and bottom electrodes for 3D cell rotation (2013)
Type of ContentConference Contributions - Other
PublisherUniversity of Canterbury. Electrical and Computer Engineering
University of Canterbury. Mechanical Engineering
AuthorsBenhal, P., Chase, J.G., Gaynor, P., Oback, B., Wang, W.show all
We report a Lab-on-chip micro device for rotating a spherical object such as bovine cell in three dimensional (3D) spaces. Micro device consists of two pairs of orthogonal vertical wall electrode along with bottom transparent indium tin oxide (ITO) electrodes. Photolithography technique is employed to fabricate bottom part of the micro device. On the other hand micro milling is applied to fabricate the top electrodes of 500 μm height. The entire device is only 75X25 mm in length and width upon which particle rotation is achieved. Micro device works entirely on this dielectrophoresis (DEP) phenomenon to rotate the cell in 3D spaces. Rotating fields are optimized through simulation and results are presented along with analysis. The experiments are carried out to determine the cell rotation rate. Rotation of cell in 3D spaces has its unique advantages in automation of high throughput mammalian cloning procedures. Cloning procedures such as enucleation requires a cell to be rotated in 3D spaces for genetic material extraction. Enucleation also requires the cell to be precisely rotated and controlled. This precise rotation control is achieved by electrorotation through DEP.
CitationBenhal, P., Chase, J.G., Gaynor, P., Oback, B., Wang, W. (2013) A micro-device integrating vertical and bottom electrodes for 3D cell rotation. Auckland, New Zealand: 6th International Conference on Advanced Materials and Technology (AMN6), 11-15 Feb 2013. 1pp.
This citation is automatically generated and may be unreliable. Use as a guide only.
KeywordsDEP; electrorotation; micro-device; micro-milling
ANZSRC Fields of Research09 - Engineering::0903 - Biomedical Engineering::090303 - Biomedical Instrumentation
09 - Engineering::0910 - Manufacturing Engineering::091009 - Microtechnology
09 - Engineering::0913 - Mechanical Engineering::091306 - Micro Electro-Mechanical Systems (MEMS)
11 - Medical and Health Sciences::1116 - Medical Physiology::111601 - Cell Physiology