Genome and transcriptome adaptation accompanying emergence of the definitive Type 2 host-restricted Salmonella enterica serovar Typhimurium pathovar (2013)
Salmonella enterica serovar Typhimurium definitive type 2 (DT2) is host restricted to Columba livia (rock or feral pigeon) but is also closely related to S. Typhimurium isolates that circulate in livestock and cause a zoonosis characterized by gastroenteritis in humans. DT2 isolates formed a distinct phylogenetic cluster within S. Typhimurium based on whole-genome-sequence polymorphisms. Comparative genome analysis of DT2 94-213 and S. Typhimurium SL1344, DT104, and D23580 identified few differences in gene content with the exception of variations within prophages. However, DT2 94-213 harbored 22 pseudogenes that were intact in other closely related S. Typhimurium strains. We report a novel in silico approach to identify single amino acid substitutions in proteins that have a high probability of a functional impact. One polymorphism identified using this method, a single-residue deletion in the Tar protein, abrogated chemotaxis to aspartate in vitro. DT2 94-213 also exhibited an altered transcriptional profile in response to culture at 42°C compared to that of SL1344. Such differentially regulated genes included a number involved in flagellum biosynthesis and motility.
CitationKingsley, R.A., Kay, S., Connor, T., Barquist, L., Sait, L., Holt, K.E., Sivaraman, K., Wileman, T., Goulding, D., Clare, S., Hale, C., Seshasayee, A., Harris, S., Thomson, N.R., Gardner, P.P., Rabsch, W., Wigley, P., Humphrey, T., Parkhill, J., Dougan, G. (2013) Genome and transcriptome adaptation accompanying emergence of the definitive Type 2 host-restricted Salmonella enterica serovar Typhimurium pathovar. American Society for Microbiology, 4(5), pp. e00565-13.
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ANZSRC Fields of Research06 - Biological Sciences::0604 - Genetics
31 - Biological sciences::3107 - Microbiology::310704 - Microbial genetics
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