Preliminary Design of a Scanning Laser Second Harmonic Generation Microscope for Collagen Imaging
Thesis DisciplineMedical Physics
Degree GrantorUniversity of Canterbury
Degree NameMaster of Science
Second Harmonic Generation (SHG) microscopy has undergone rapid transformation as an imaging tool for research due to its noninvasive properties that provides high resolution images for quantitative analysis. Extensive research has been performed to study the type of materials compatible with SHG but the effects of varying laser wavelengths on SHG efficiency is still poorly understood. The aim of this project is to design and build a scanning laser SHG microscope that is optimised for a 1030 nm giant chirp oscillator (GCO) designed by the University of Auckland. The project has shown that this wavelength is capable of producing SHG in collagen tissues of thickness up to 100 µm. We used nonlinear crystals and collagen samples to acquire signals and high resolution 2D images. The images were found to be of excellent quality with resolutions of up to a few microns to allow accurate measurements of the size and orientation of the fibrillar structures.