Non-invasive characterization of dissolved oxygen dynamics in water-in-oil droplet microfluidics – Towards 3d micro tumor spheroids for high throughput cancer drug screening (2013)
Type of ContentConference Contributions - Published
PublisherUniversity of Canterbury. Electrical and Computer Engineering
University of Canterbury. Biomolecular Interaction Centre
AuthorsErhardt, J., Nock, V., Kieninger, J., Urban, G.A.show all
Oxygenation is a key parameter in tumor spheroid culturing. This work reports on the study of oxygen concentration and gas exchange dynamics in two-phase microfluidic droplet systems. Initial measurements of oxygen in single, oil-covered aqueous droplets in micro-wells show the feasibility of droplet-based sensing. We further introduce on-chip generation and trapping of individual droplets with controlled dissolved oxygen concentrations. Luminescence-based optical sensing is used to characterize the time-resolved dynamics of gas exchange between surrounding oil and micro-droplets where both phases exhibit pre-defined oxygen concentrations. This will help to determine suitable fluid combinations for oxygen-sensitive applications, such as high-throughput screening of drugs on micro-spheroids.
CitationErhardt, J., Nock, V., Kieninger, J., Urban, G.A. (2013) Non-invasive characterization of dissolved oxygen dynamics in water-in-oil droplet microfluidics – Towards 3d micro tumor spheroids for high throughput cancer drug screening. Freiburg, Germany: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), 27-31 Oct 2013. 1376-1378.
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KeywordsMicrofluidic droplets; oxygen measurements; Cancer spheroids; RTDP
ANZSRC Fields of Research03 - Chemical Sciences::0304 - Medicinal and Biomolecular Chemistry::030402 - Biomolecular Modelling and Design
09 - Engineering::0903 - Biomedical Engineering::090303 - Biomedical Instrumentation