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    Investigating the substrate specificity of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) synthase (2011)

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    Type of Content
    Theses / Dissertations
    UC Permalink
    http://hdl.handle.net/10092/6565
    http://dx.doi.org/10.26021/6668
    Thesis Discipline
    Chemistry
    Degree Name
    Doctor of Philosophy
    Publisher
    University of Canterbury. Chemistry
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    • Science: Theses and Dissertations [3780]
    Authors
    Tran, Davidshow all
    Abstract

    The shikimate pathway is a biosynthetic pathway that is responsible for producing a variety of organic compounds that are necessary for life in plants and microorganisms. The pathway consists of seven enzyme catalysed reactions beginning with the condensation reaction between D-erythrose 4-phosphate (E4P) and phosphoenolpyruvate (PEP) to give the seven-carbon sugar DAH7P. This thesis describes the design, synthesis and evaluation of a range of alternative non-natural four-carbon analogues of E4P (2- and 3-deoxyE4P, 3-methylE4P, phosphonate analogues of E4P) to probe the substrate specificity of different types of DAH7P synthases [such as Mycobacterium tuberculosis (a type II DAH7PS), Escherichia coli (a type Ialpha DAH7PS) and Pyrococcus furiosus (a type Ibeta DAH7PS)].

    Keywords
    DAH7PS; substrate specificity; erythrose 4-phosphate; aromatic amino acid biosynthesis; shikimate pathway
    Rights
    Copyright David Tran
    https://canterbury.libguides.com/rights/theses
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