Drug analysis by mass spectrometry
Degree GrantorUniversity of Canterbury
Degree NameDoctor of Philosophy
1. The pharmacokinetics of morphine have been measured in four patients with renal failure and three healthy volunteers following intramuscular administration of papaveretum. Morphine blood levels were determined using GCMS with specific ion monitoring. The significantly shorter drug elimination half-life found for the patients suggests that renal failure does not impair the elimination of morphine. 2. A CI GCMS assay with specific ion monitoring has been developed for measuring the anti parkinsonian drug benztropine in post-mortem specimens. The assay is potentially more sensitive than a similar assay using electron impact ionisation. 3. The level of atropine in an aqueous extract of Datura stramonium has been measured by GCMS with selected ion monitoring. The results show that such an extract will contain most of the atropine present in the plant material. The levels are such that several medium sized glassfuls will contain sufficient atropine to constitute a dangerous drug dose. 4. A Hewlett-Packard 5982A GCMS has been successfully modified to allow it to be used to record in-beam mass spectra with a commercially available DCI probe. The effectiveness of these modifications is discussed and in-beam mass spectra of some physiologically active compounds are presented.