Spatially-Resolved Measurement of Dissolved Oxygen in Multi-Stream Microfluidic Devices (2010)
Type of ContentJournal Article
PublisherUniversity of Canterbury. Electrical and Computer Engineering
Dissolved oxygen (DO) is an important parameter with significant effect on cellular development and function. Micron-scale laminar flow and hydrodynamic focusing provide ideal tools for the generation of controlled chemical microenvironments and their application as stimuli to cells. In this paper we demonstrate the generation and characterization of multi-stream laminar flow and hydrodynamically focused sample streams with defined dissolved oxygen concentrations on chip. A solid-state oxygen sensor layer was integrated into PDMS-based microchannels and calibrated. Several combinations of sample and buffer streams with concentrations ranging from 0 to 34 mg/l DO were generated and measured for up to three independent parallel flow streams. In addition, diffusion-based stream broadening measured with the sensor was used to determine the coefficient of diffusion of O2 in the flow medium. The devices have the potential to provide novel insights into cell biology and improve the relevance of in-vitro cell assays.
CitationNock, V., Blaikie, R.J. (2010) Spatially-Resolved Measurement of Dissolved Oxygen in Multi-Stream Microfluidic Devices. IEEE Sensors Journal, 10(12), pp. 1813-1819.
This citation is automatically generated and may be unreliable. Use as a guide only.
Keywordsmicrofluidics; hydrodynamic focusing; optical oxygen sensor; PtOEPK/PS; spatial measurement
ANZSRC Fields of Research06 - Biological Sciences::0601 - Biochemistry and Cell Biology
Showing items related by title, author, creator and subject.
Non-invasive characterization of dissolved oxygen dynamics in water-in-oil droplet microfluidics – Towards 3d micro tumor spheroids for high throughput cancer drug screening Erhardt, J.; Nock, V.; Kieninger, J.; Urban, G.A. (University of Canterbury. Electrical and Computer EngineeringUniversity of Canterbury. Biomolecular Interaction Centre, 2013)Oxygenation is a key parameter in tumor spheroid culturing. This work reports on the study of oxygen concentration and gas exchange dynamics in two-phase microfluidic droplet systems. Initial measurements of oxygen in ...
Nock, Volker; Onal, Sevgi; Alkaisi, Maan (2021)
Orcheston-Findlay, Louise; Hashemi, Azadeh; Garrill, Ashley; Nock, Volker (Elsevier BV, 2018)© 2018 Elsevier B.V. Standard cancer lab models lack many attributes of the in-vivo cancer microenvironment. Oxygen levels for example are not commonly controlled in 2D cell-culture well plate experiments. However, low O2 ...