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    Image analysis for electron microscopy of muscle fibres

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    12602383_image_analysis.pdf (443.8Kb)
    Author
    Yoon, C.H.
    Bones, P.J.
    Millane, R.P.
    Date
    2005
    Permanent Link
    http://hdl.handle.net/10092/3351

    An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined by filtering with a point spread function that incorporates the local symmetry in an image. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and the orientations classified using a Gaussian mixture model. Application to micrographs and comparison with manual classification of orientations shows that the system is effective.

    Subjects
    Fields of Research::290000 Engineering and Technology::290900 Electrical and Electronic Engineering::290903 Other electronic engineering
     
    Fields of Research::320000 Medical and Health Sciences::321000 Clinical Sciences::321022 Radiology and organ imaging
     
    Fields of Research::320000 Medical and Health Sciences::329900 Other Medical and Health Sciences::329902 Medical biotechnology
    Collections
    • Engineering: Conference Contributions [1920]
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