Image analysis for electron microscopy of muscle fibres (2005)
AuthorsYoon, C.H., Bones, P.J., Millane, R.P.show all
An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined by filtering with a point spread function that incorporates the local symmetry in an image. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and the orientations classified using a Gaussian mixture model. Application to micrographs and comparison with manual classification of orientations shows that the system is effective.
CitationYoon, C.H., Bones, P.J. and Millane, R.P. (2005) Image analysis for electron microscopy of muscle fibres. Cairns, Australia: Digital Image Computing: Techniques and Applications, 6-8 Dec 2005. Proceedings, CD-Rom, 79/1-7.
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