Micro-patterning of polymer-based optical oxygen sensors for lab-on-chip applications (2007)

Type of Content
Conference Contributions - PublishedPublisher
University of Canterbury. Electrical and Computer EngineeringUniversity of Canterbury. Mechanical Engineering
Collections
Abstract
Soft-lithography and plasma etching with reactive ions were used to fabricate a polymer microfluidic cell-culture bioreactor with integrated optical oxygen sensor. Platinum (II) octaethylporphyrin ketone (PtOEPK) suspended in a microporous polystyrene (PS) matrix was spin-coated to form sensor films of variable thickness from 1.1 µm to 400 nm on glass substrates. Sensor films were found to be smooth and well adhered. Arbitrary patterns with a minimum feature size of 25 µm could be routinely replicated in the PtOEPK/PS layer using polydimethylsiloxane (PDMS) elastomer stamps as etch masks in a reactive ion etcher. No effect of plasma patterning and sensor integration by plasma bonding on the sensor signal could be observed. Detection of different gaseous and dissolved oxygen concentrations with the patterned sensor followed linear Stern-Volmer behavior. Dynamic measurement of sensor intensity as a function of different oxygen concentration showed good reproducibility and a nearly instantaneous response to gas changes. For gaseous and dissolved oxygen measurement with a patterned 400 nm thick film I0/I100 ratios of 3.2 and 2.7 were found, respectively.
Citation
Nock, V.M., Blaikie, R.J., David, T. (2007) Micro-patterning of polymer-based optical oxygen sensors for lab-on-chip applications. Canberra, Australia: Microelectronics, MEMS, and Nanotechnology 2007: BioMEMS and Nanotechnology III Conference 6799, 5-7 Dec 2007. Proceedings of SPIE, 6799, BioMEMS and Nanotechnology III, 67990Y.This citation is automatically generated and may be unreliable. Use as a guide only.
Keywords
optical oxygen sensor; PtOEPK in PS; micro-patterning; RIE; sensor integration; fluorescence microscopyRelated items
Showing items related by title, author, creator and subject.
-
Patterning, integration and characterisation of polymer optical oxygen sensors for microfluidic devices
Nock, V.M.; Blaikie, R.J.; David, T. (University of Canterbury. Electrical and Computer EngineeringUniversity of Canterbury. Mechanical Engineering, 2008)This paper describes a process for the layer-by-layer fabrication and integration of luminescent dye-based optical oxygen sensors into microfluidic devices. Application of oxygen-sensitive platinum(II) octaethylporphyrin ... -
Oxygen Control For Bioreactors And In-vitro Cell Assays
Nock, V.; Blaikie, R.J.; David, T. (University of Canterbury. Electrical and Computer EngineeringUniversity of Canterbury. Mechanical Engineering, 2009)Dissolved oxygen (DO) is an important parameter in biomedical and cell-culture applications. Several studies have found cell survival and function to be intimately linked to oxygen concentration. Laminar flow, as observed ... -
A microfluidic gradient generator to simulate the oxygen microenvironment in cancer cell culture
Orcheston-Findlay, Louise; Hashemi, Azadeh; Garrill, Ashley; Nock, Volker (Elsevier BV, 2018)© 2018 Elsevier B.V. Standard cancer lab models lack many attributes of the in-vivo cancer microenvironment. Oxygen levels for example are not commonly controlled in 2D cell-culture well plate experiments. However, low O2 ...