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    Plant-produced cottontail rabbit papillomavirus L1 protein protects against tumor challenge: a proof-of-concept study

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    Author
    Kohl, T.
    Hitzeroth, I.I.
    Stewart, D.
    Varsani, A.
    Govan, V.A.
    Christensen, N.D.
    Williamson, A.L.
    Rybicki, E.P.
    Date
    2006
    Permanent Link
    http://hdl.handle.net/10092/2387

    The native cottontail rabbit papillomavirus (CRPV) L1 capsid protein gene was expressed transgenically via Agrobacterium tumefaciens transformation and transiently via a tobacco mosaic virus (TMV) vector in Nicotiana spp. L1 protein was detected in concentrated plant extracts at concentrations up to 1.0 mg/kg in transgenic plants and up to 0.4 mg/kg in TMV-infected plants. The protein did not detectably assemble into viruslike particles; however, immunoelectron microscopy showed presumptive pentamer aggregates, and extracted protein reacted with conformation-specific and neutralizing monoclonal antibodies. Rabbits were injected with concentrated protein extract with Freund's incomplete adjuvant. All sera reacted with baculovirus-produced CRPV L1; however, they did not detectably neutralize infectivity in an in vitro assay. Vaccinated rabbits were, however, protected against wart development on subsequent challenge with live virus. This is the first evidence that a plant-derived papillomavirus vaccine is protective in an animal model and is a proof of concept for human papillomavirus vaccines produced in plants.

    Subjects
    virus-like particles
     
    cervical-cancer
     
    young-women
     
    immunization
     
    type-16
     
    expression
     
    vaccine
     
    vector
     
    crpv
     
    neutralization
     
    Fields of Research::270000 Biological Sciences
     
    Fields of Research::270000 Biological Sciences::270300 Microbiology::270303 Virology
    Collections
    • Science: Journal Articles [912]
    Rights
    https://hdl.handle.net/10092/17651

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