A monolithic polydimethylsiloxane platform for zoospore Capture, germination and single hypha force sensing (2019)
AuthorsSun Y, Tayagui A, Garrill A, Nock Vshow all
This paper reports a triple-layer, polydimethylsiloxane (PDMS)-based lab-on-a-chip platform combining the capture and culture of individual oomycete zoospores with integrated force sensing on germinated hyphae. The platform enables the concurrent study of cell-to-cell variability in hyphal growth and protrusive force generation. To demonstrate the applicability of the platform, individual zoospores of the oomycete Achlya bisexualis were trapped by a constriction structure, cultured on the device and the micro-Newton forces exerted by hyphae measured by tracking the deflection of elastomeric micropillars. The platform provides a new tool to help understand protrusive growth on a single cell level.
CitationSun Y, Tayagui A, Garrill A, Nock V (2019). A monolithic polydimethylsiloxane platform for zoospore Capture, germination and single hypha force sensing. Berlin, Germany: The 20th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers). 23/06/2019-27/06/2019. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII). 409-412.
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KeywordsLab-on-a-Chip; Single Cell Analysis; Membrane Valve; Force Sensor; Fungi; Oomycetes
ANZSRC Fields of Research06 - Biological Sciences::0605 - Microbiology::060505 - Mycology
10 - Technology::1007 - Nanotechnology::100703 - Nanobiotechnology