A monolithic polydimethylsiloxane platform for zoospore Capture, germination and single hypha force sensing
This paper reports a triple-layer, polydimethylsiloxane (PDMS)-based lab-on-a-chip platform combining the capture and culture of individual oomycete zoospores with integrated force sensing on germinated hyphae. The platform enables the concurrent study of cell-to-cell variability in hyphal growth and protrusive force generation. To demonstrate the applicability of the platform, individual zoospores of the oomycete Achlya bisexualis were trapped by a constriction structure, cultured on the device and the micro-Newton forces exerted by hyphae measured by tracking the deflection of elastomeric micropillars. The platform provides a new tool to help understand protrusive growth on a single cell level.