Cell organelles and fluorescence of parenchyma cells in Eucalyptus bosistoana sapwood and heartwood investigated by microscopy (2018)
Type of ContentJournal Article
Background: Eucalyptus bosistoana is currently investigated in New Zealand for its potential to produce naturally durable timber in short-rotation plantations. Little is known of heartwood formation in young trees. The objective of this study was to identify conventional and confocal microscopy methods which allow the observation of cell organelles and the chemical composition in the E. bosistoana parenchyma cells before and after heartwood formation. Results: Nuclei, microtubules and peroxisomes in parenchyma cells of 2-year-old E. bosistoana stems were visualised by confocal microscopy combined with optimised immunolabelling protocols. Sequential staining of the tissue with toluidine blue and iodine/potassium iodide identified different cell organelles in parenchyma cells of sapwood. Iodine/ potassium iodide stained starch (amyloplasts), while amido black stained proteins in sapwood. Fluorescence emission spectra confirmed the presence of chloroplasts in parenchyma of 2-year-old E. bosistoana. Fluorescence emission spectral (lambda) scans showed differences between parenchyma and fibre cells as well as sapwood and heartwood. Conclusions: Physiological changes between sapwood and heartwood were visualised in parenchyma cells. Labelling of cell organelles was challenging due to unspecific binding and high background signals. Understanding heartwood formation is critical for the success of a plantation forest industry aiming to produce ground-durable timber, as heartwood formation is variable.
KeywordsAmyloplasts; Chloroplasts; Confocal microscopy; Extractives; Microtubules; Nuclei; Parenchyma cells; Wood (xylem)
ANZSRC Fields of Research06 - Biological Sciences::0607 - Plant Biology::060702 - Plant Cell and Molecular Biology
07 - Agricultural and Veterinary Sciences::0705 - Forestry Sciences
Rights© The Author(s). 2018 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made
Showing items related by title, author, creator and subject.
Mishra G; Garrill A; Altaner CM (Italian Society of Sivilculture and Forest Ecology (SISEF), 2019)Variability in bioactivity and chemical composition of Eucalyptus bosistoana F. Muell. heartwood extracts between individual trees from two different sites were investigated. Combining the results of fungal assays and ...
Effects of variable selection and processing of NIR and ATR-IR spectra on prediction of extractives content in Eucalyptus bosistoana heartwood Li Y; Altaner CM (2019)The use of quick attenuated total reflectance infrared (ATR-IR) spectroscopy and near infrared (NIR) spectroscopy to predict extractives content (EC) in heartwood of E. bosistoana with partial least squares regression (PLSR) ...
Li, Y.; Altaner, C. (University of Canterbury. School of Forestry, 2015)