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    Passion fruit chlorotic mottle virus: Molecular characterization of a new divergent geminivirus in Brazil (2018)

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    Type of Content
    Journal Article
    UC Permalink
    http://hdl.handle.net/10092/16371
    
    Publisher's DOI/URI
    https://doi.org/10.3390/v10040169
    
    ISSN
    1999-4915
    1999-4915
    Language
    English
    Collections
    • Science: Journal Articles [1118]
    Authors
    Fontenele RS
    Abreu RA
    Lamas NS
    Alves-Freitas DMT
    Vidal AH
    Poppiel RR
    Melo FL
    Lacorte C
    Martin DP
    Campos MA
    Varsani A
    Ribeiro SG
    show all
    Abstract

    © 2018 by the authors. Licensee MDPI, Basel, Switzerland. Brazil is one of the major passion fruit producers worldwide. Viral diseases are among the most important constraints for passion fruit production. Here we identify and characterize a new passion fruit infecting-virus belonging to the family Geminiviridae: passion fruit chlorotic mottle virus (PCMoV). PCMoV is a divergent geminivirus unlike previously characterized passion fruit-infecting geminiviruses that belonged to the genus Begomovirus. Among the presently known geminiviruses, it is most closely related to, and shares ~62% genome-wide identity with citrus chlorotic dwarf associated virus (CCDaV) and camelia chlorotic dwarf associated virus (CaCDaV). The 3743 nt PCMoV genome encodes a capsid protein (CP) and replication-associated protein (Rep) that respectively share 56 and 60% amino acid identity with those encoded by CaCDaV. The CPs of PCMoV, CCDaV, and CaCDaV cluster with those of begomovirus whereas their Reps with those of becurtoviruses. Hence, these viruses likely represent a lineage of recombinant begomo-like and becurto-like ancestral viruses. Furthermore, PCMoV, CCDaV, and CaCDaV genomes are ~12–30% larger than monopartite geminiviruses and this is primarily due to the encoded movement protein (MP; 891–921 nt) and this MP is most closely related to that encoded by the DNA-B component of bipartite begomoviruses. Hence, PCMoV, CCDaV, and CaCDaV lineage of viruses may represent molecules in an intermediary step in the evolution of bipartite begomoviruses (~5.3 kb) from monopartite geminiviruses (~2.7–3 kb). An infectious clone of PCMoV systemically infected Nicotiana benthamina, Arabidopsis thaliana, and Passiflora edulis.

    Keywords
    geminivirus; passion fruit; Passiflora sp.
    ANZSRC Fields of Research
    31 - Biological sciences::3101 - Biochemistry and cell biology::310102 - Cell development, proliferation and death
    Rights
    © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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