Synthesis and application of gold and glycogold nanoparticles.
Degree GrantorUniversity of Canterbury
Degree NameDoctor of Philosophy
Gold nanoparticles have drawn significant interest in recent years due to their unique optical, electronic, and molecular recognition properties. It is widely known that the key properties of gold nanoparticles are dependent on their size, morphology, and nature of the ligands that stabilize them against aggregation. A recent development in the field of gold nanoparticle chemistry is the synthesis and application of glycogold nanoparticles, which are water-soluble three-dimensional multivalent systems based on carbohydrate decorated gold nanoparticles. Glycogold nanoparticles are typically synthesized by attaching carbohydrates to the surface of gold nanoparticles via thiol-terminated linkers. They have been used to detect many toxic proteins such as concanavalin A, Ricinus communis agglutinin 120, cholera toxin, and viral hemagglutinin. Facile methods for the synthesis of gold and glycogold nanoparticles and the demonstration of the efficient use of glycogold nanoparticles as colorimetric sensors for toxic proteins are highly sought after. The work in this thesis focuses on the synthesis of gold nanoparticles of various shapes and sizes using the Turkevich reaction and the synthesis of glycogold nanoparticles using Brust-Schiffrin method, ligand exchange method, and the copper(I)-catalysed azide alkyne Huisgen cycloaddition. Also, the application of galactose-capped gold nanoparticles and full-length sialic acid terminated complex bi-antennary N-glycan capped gold nanoparticles as colorimetric sensors for the detection of the lectin heat labile enterotoxin and influenza viral particles respectively has been presented.