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    Physical modelling of electroporation in close cell-to-cell proximity environments

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    Author
    Gaynor, P.T.
    Bodger, P.S.
    Date
    2006
    Permanent Link
    http://hdl.handle.net/10092/116

    Many applications of electroporation, especially those utilizing electrofusion and in-vivo electroporation, involve cell environments that include close cell-to-cell proximity and a wide range of target cell size. It is important to understand how this kind of environment may alter optimum electroporation electrical parameters for any given application. A physical, electrically equivalent model of biological cell electroporation, based on aqueous solution filled thin latex rubber membrane spheroids, was used to investigate membrane permeabilization behaviour where there is both close cell-to-cell proximity and different cell radii. Cell model arrangements were pulsed using either a 50 µs or 10 µs, 1/e decay time constant dc capacitive discharge electric field, with peak amplitudes of 160-500 kV m⁻¹. Results indicate that, compared to cells in isolation, electroporation initiates at substantially decreased applied electric field magnitudes in regions of close cell-to-cell proximity where the external media conductivity is lower than the cell interior conductivity, and the membrane is maximally polarized. Additionally, the use of shorter time constant, higher peak magnitude pulse parameters should reduce the relative difference in threshold membrane permeabilization in regions of close cell-to-cell proximity for cells of different size so that the degree of electroporation is more uniform for variable size and shape target cell populations.

    Subjects
    electroporation
     
    electropermeabilization
     
    physical modelling
     
    in-vivo
     
    pulsed
     
    Fields of Research::290000 Engineering and Technology::290900 Electrical and Electronic Engineering::290901 Electrical engineering
     
    Fields of Research::290000 Engineering and Technology::291500 Biomedical Engineering::290599 Biomedical engineering not elsewhere classified
    Collections
    • Engineering: Journal Articles [1124]
    Rights
    https://hdl.handle.net/10092/17651

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