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    Microfluidic Platform to Study Electric Field Based Root Targeting by Pathogenic Zoospores (2022)

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    Type of Content
    Conference Contributions - Published
    UC Permalink
    https://hdl.handle.net/10092/103667
    
    Publisher's DOI/URI
    http://doi.org/10.1109/mems51670.2022.9699559
    
    Publisher
    IEEE
    Collections
    • BIC: Conference Contributions [2]
    Authors
    Sarkar D
    Sun Y
    Tayagui A
    Adams R
    Garrill A
    Nock, Volker cc
    show all
    Abstract

    This paper reports the fabrication and application of a microfluidic Lab-on-a-Chip platform to study the electrotactic movements of pathogenic microorganisms. The movement of the pathogens in response to electric fields are one way in which they are thought to locate their hosts. Design and fabrication of the platform, and associated micro-electronics are described. The platform contains arrays of micro-electrodes that generate an electric field of defined strength in a micro-chamber into which feed inlet and outlet channels for entry and exit of media and microorganisms. To demonstrate applicability of the platform, motile zoospores of the pathogenic oomycete Phytophthora nicotianae were seeded in the inlet and a voltage was applied to investigate the electrotactic responses of the zoospores. This platform offers a unique opportunity to study electrotactic movements that may be responsible for the ability of the pathogens to locate and invade host tissue.

    Citation
    Sarkar D, Sun Y, Tayagui A, Adams R, Garrill A, Nock V (2022). Microfluidic Platform to Study Electric Field Based Root Targeting by Pathogenic Zoospores. 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS). 09/01/2022-13/01/2022. 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS).
    This citation is automatically generated and may be unreliable. Use as a guide only.
    Keywords
    Phytophthora nicotianae; Zoospores; Electrotaxis; Electric Fields; Lab-on-a-Chip
    ANZSRC Fields of Research
    40 - Engineering::4012 - Fluid mechanics and thermal engineering::401210 - Microfluidics and nanofluidics
    40 - Engineering::4009 - Electronics, sensors and digital hardware::400908 - Microelectronics
    31 - Biological sciences::3107 - Microbiology::310705 - Mycology
    Rights
    All rights reserved unless otherwise stated
    http://hdl.handle.net/10092/17651

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