Microfluidic Platform to Study Electric Field Based Root Targeting by Pathogenic Zoospores (2022)

View/ Open
Type of Content
Conference Contributions - PublishedPublisher
IEEECollections
Abstract
This paper reports the fabrication and application of a microfluidic Lab-on-a-Chip platform to study the electrotactic movements of pathogenic microorganisms. The movement of the pathogens in response to electric fields are one way in which they are thought to locate their hosts. Design and fabrication of the platform, and associated micro-electronics are described. The platform contains arrays of micro-electrodes that generate an electric field of defined strength in a micro-chamber into which feed inlet and outlet channels for entry and exit of media and microorganisms. To demonstrate applicability of the platform, motile zoospores of the pathogenic oomycete Phytophthora nicotianae were seeded in the inlet and a voltage was applied to investigate the electrotactic responses of the zoospores. This platform offers a unique opportunity to study electrotactic movements that may be responsible for the ability of the pathogens to locate and invade host tissue.
Citation
Sarkar D, Sun Y, Tayagui A, Adams R, Garrill A, Nock V (2022). Microfluidic Platform to Study Electric Field Based Root Targeting by Pathogenic Zoospores. 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS). 09/01/2022-13/01/2022. 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS).This citation is automatically generated and may be unreliable. Use as a guide only.
Keywords
Phytophthora nicotianae; Zoospores; Electrotaxis; Electric Fields; Lab-on-a-ChipANZSRC Fields of Research
40 - Engineering::4012 - Fluid mechanics and thermal engineering::401210 - Microfluidics and nanofluidics40 - Engineering::4009 - Electronics, sensors and digital hardware::400908 - Microelectronics
31 - Biological sciences::3107 - Microbiology::310705 - Mycology
Rights
All rights reserved unless otherwise statedRelated items
Showing items related by title, author, creator and subject.
-
Lab-on-a-chip Platforms for Single Zoospore, Fungal and Oomycete Studies
Sun Y; Tayagui A; Garrill A; Nock, Volker (2021) -
On-chip measurement of protrusive force exerted by single hyphal tips of pathogenic microorganisms
Tayagui A; Garrill A; Collings D; Nock VM (2016)Invasive growth is a process used by fungi and oomycetes to find sources of nutrients. To study the protrusive forces generated as part of this process, we have developed a Lab-on-a-Chip platform capable of measuring ... -
Hybrid normally closed dry-film/elastomer valves for integrated electrotaxis studies
Sun Y; Sarkar D; Adams R; Tayagui A; Garrill A; Nock, Volker (2021)This paper presents the fabrication and characterization of novel normally closed hybrid dry-film/elastomer membrane valves for the integration of electrode arrays into lab-on-a-chip platforms for electrotaxis studies. ...