First Responders to Cataclysmic Upheaval: Earthquake–Driven Effects on Microalgae in the Avon-Heathcote Estuary, Christchurch, New Zealand.

Abstract

The Avon-Heathcote Estuary is of significant value to Christchurch due to its high productivity, biotic diversity, proximity to the city, and its cultural, recreational and aesthetic qualities. Nonetheless, it has been subjected to decades of degradation from sewage wastewater discharges and encroaching urban development. The result was a eutrophied estuary, high in nitrogen, affected by large blooms of nuisance macroalgae and covered by degraded sediments. In March 2010, treated wastewater was diverted from the estuary to a site 3 km offshore. This quickly reduced water nitrogen by 90% within the estuary and, within months, there was reduced production of macroalgae. However, a series of earthquakes beginning in September 2010 brought massive changes: tilting of the estuary, changes in channels and water flow, and a huge influx of liquefied sediments that covered up to 65% of the estuary floor. Water nitrogen increased due to damage to sewage infrastructure and the diversion pipeline being turned off. Together, these drastically altered the estuarine ecosystem. My study involves three laboratory and five in situ experiments that investigate the base of the food chain and responses of benthic microalgae to earthquake-driven sediment and nutrient changes. It was predicted that the new sediments would be coarser and less contaminated with organic matter and nutrients than the old sediments, would have decreased microalgal biomass, and would prevent invertebrate grazing and bioturbation activities. It was believed that microalgal biomass would become similar across new and old sediments types as the unstable new sediments were resuspended and distributed over the old sediments. Contact cores of the sediment were taken at three sites, across a eutrophication gradient, monthly from September 2011 to March 2012. Extracted chlorophyll a pigments showed that microalgal biomass was generally lower on new liquefied sediments compared to old sediments, although there was considerable site to site variation, with the highly eutrophic sites being the most affected by the emergence of the new sediments. Grazer experiments showed that invertebrates had both positive and negative site-specific effects on microalgal biomass depending on their identity. At one site, new sediments facilitated grazing by Amphibola crenata, whereas at another site, new sediments did not alter the direct and indirect effects of invertebrates (Nicon aestuariensis, Macropthalmus hirtipes, and A. crenata) on microalgae. From nutrient addition experiments it was clear that benthic microalgae were able to use nutrients from within both old and new sediments equally. This implied that microalgae were reducing legacy nutrients in both sediments, and that they are an important buffer against eutrophication. Therefore, in tandem with the wastewater diversion, they could underpin much of the recovery of the estuary. Overall, the new sediments were less favourable for benthic microalgal growth and recolonisation, but were less contaminated than old sediments at highly eutrophic sites. Because the new sediments were less contaminated than the old sediments, they could help return the estuary to a noneutrophic state. However, if the new sediments, which are less favourable for microalgal growth, disperse over the old sediments at highly eutrophic sites, they could become contaminated and interfere with estuarine recovery. Therefore, recovery of microalgal communities and the estuary was expected to be generally long, but variable and site-specific, with the least eutrophic sites recovering quickly, and the most eutrophic sites taking years to return to a pre-earthquake and non-eutrophied state. changes in channels and water flow, and a huge influx of liquefied sediments that covered up to 65% of the estuary floor. Water nitrogen increased due to damage to sewage infrastructure and the diversion pipeline being turned off. Together, these drastically altered the estuarine ecosystem. My study involves three laboratory and five in situ experiments that investigate the base of the food chain and responses of benthic microalgae to earthquake-driven sedimen tand nutrient changes. It was predicted that the new sediments would be coarser and less contaminated with organic matter and nutrients than the old sediments, would have decreased microalgal biomass, and would prevent invertebrate grazing and bioturbation activities. It was believed that microalgal biomass would become similar across new and old sediments types as the unstable new sediments were resuspended and distributed over the old sediments. Contact cores of the sediment were taken at three sites, across a eutrophication gradient, monthly from September 2011 to March 2012. Extracted chlorophyll a pigments showed that microalgal biomass was generally lower on new liquefied sediments compared to old sediments, although there was considerable site to site variation, with the highly eutrophic sites being the most affected by the emergence of the new sediments. Grazer experiments showed that invertebrates had both positive and negative site-specific effects on microalgal biomass depending on their identity. At one site, new sediments facilitated grazing by Amphibola crenata, whereas at another site, new sediments did not alter the direct and indirect effects of invertebrates (Nicon aestuariensis, Macropthalmus hirtipes, and A. crenata) on microalgae. From nutrient addition experiments it was clear that benthic microalgae were able to use nutrients from within both old and new sediments equally. This implied that microalgae were reducing legacy nutrients in both sediments, and that they are