Behavioural effects of grafts of two areas of the basal forebrain and their combination after fimbria-fornix lesion (1995)
Type of ContentTheses / Dissertations
Degree NameMaster of Science
PublisherUniversity of Canterbury
Adult rats received bilateral fimbria-fornix/cingulate cortex lesions followed by intrahippocampal (12mm CRL) suspensions of either Lateral Basal Forebrain (LBF), Ventromedial Basal Forebrain (VBF) or a mixture of both tissues (group MBF), equated for cell density. Relative to lesion-only controls, there was no amelioration in any graft group of the lesion-induced working memory impairment in a T-maze task. Postoperative reacquisition of reference memory performance, however, was significantly impaired only in group LBF by comparison with intact controls; the other three lesion groups showed an intermediate level of performance. By comparison with intact controls, all four lesion groups were also impaired on DRL-20 and subsequent DRL-10 tests. Unlike the DRL-20 test, lesioned rats showed improvement across sessions on the DRL-10 test; relative to intact controls, the lesion-only group's final performance on DRL-10 was not significantly impaired whereas as all three grafted groups remained significantly impaired throughout training. Lesion-induced spontaneous hyperactivity was not affected by graft status. Surviving implants were found in all grafted rats, with moderate to excellent cholinergic reinnervation present in groups VBF and MBF. However, weak or virtually absent cholinergic reinnervation was present in group LBF, with relatively poor AChE-positivity outside the region of the graft tissue. This study provides further evidence that basal forebrain grafts, irrespective of any associated cholinergic innervation of the hippocampus, may not improve lesion-induced behavioural deficits.
KeywordsNerve grafting; Nervous system--Regeneration; Nerve tissue--Transplantation; Central nervous system--Pathophysiology; Central nervous system--Diseases--Animal models
RightsAll Rights Reserved
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